FIGURE

Fig. S10

ID

ZDB-FIG-190920-6

Publication

Liu et al., 2019 - Enhanced Cas12a editing in mammalian cells and zebrafish

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Fig. S10

Aggregate nuclease activity for LbCas12a RNP complexed with the three different crRNAs across ten different target sites with or without heatshock. Heatshock increases the median indel rates for all three crRNA frameworks. The highest median levels of nuclease activity are observed with the DRf-GC@13-crRNA. Deep sequencing data are from three independent zebrafish embryo injections performed by three different individuals. Each Box plot is drawn by GraphPad Prism, where the box represents the 25th and 75th percentile and the middle line is the median. Statistical significance is determined by one-way analysis of variance (ANOVA), “*” denotes P <0.05, “ns” denotes no significance (Supplementary Table 7). (B) Phenotypes obtained after the injection of the LbCas12a-crRNA RNP complexes targeting slc45a2 (albino) showing a strong albino phenotype (lower panel) compared to the WT uninjected animals (upper panel).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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