FIGURE

Fig. 3

ID

ZDB-FIG-190918-13

Publication

Masud et al., 2019 - Rubicon-Dependent Lc3 Recruitment to Salmonella-Containing Phagosomes Is a Host Defense Mechanism Triggered Independently From Major Bacterial Virulence Factors

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Fig. 3

Host LAP response to S. Typhimurium mutants in Rubicon-depleted embryos. (A) Work flow and time line of experiments in (B–K). (B,D,F,H,J) Representative confocal micrographs of GFP-Lc3 positive infected phagocytes with mCherry-labeled S. Typhimurium mutant strains ΔphoP (B), ΔpurA (D), ΔsipB (F), ΔssrB(H), and ΔflhD (J) in Rubicon-depleted hosts (B',D',F',H',J') along with respective controls (B,D,F,H,J). (C,E,G,I,K) Quantification of GFP-Lc3-Salmonella association for S. Typhimurium mutant strains ΔphoP (C), ΔpurA (E), ΔsipB (G), ΔssrB (I), and ΔflhD (K) in Rubicon-depleted larvae and their respective controls. Numbers of infected phagocytes positive or negative for GFP-Lc3-Salmonella associations were counted from confocal images and the percentages of Lc3-positive over the total were averaged from five embryos per group. Error bars represent the SD. One of the two replicates is shown. Scale bar (B,D,F,H,J) = 10 μm, ^****P< 0.0001, ^***P < 0.001, ^**P < 0.01, ^*P < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Infect Microbiol