FIGURE

Fig. 2

ID

ZDB-FIG-190822-12

Publication

Liang et al., 2019 - Overexpression of Notch Signaling Induces Hyperosteogeny in Zebrafish

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Fig. 2

Evaluation of calcification in N1aICD-expressed transgenic zebrafish. (A–D) Calcein staining on wild type and Tg(Ola.Sp7:N1aICD)^cy31 embryos at 7 days post-fertilization (dpf). The * indicates the formed column. (E) Quantification of calcification degree by calculating the area of ring centrum formation in the notochord. N represents the fish number used for analysis. (F,G,H) EGFP-expressed osteoblasts in green fluorescence, alizarin complexone (ALC) staining region and merged image in notochord of Tg(Ola.Sp7:EGFP)^cy25 transgenic fish aged at 21 dpf. (I,J,K) N1aICD-expressed osteoblasts in green fluorescence, ALC staining region and merged image in notochord of double transgenic fish of Tg(Ola.Sp7:EGFP)^cy25 and Tg(Ola.Sp7:N1aICD)^cy31 at 21 dpf. The dashed line surrounded areas were measured using image quantitation and summed up for comparison. (L,M,N) EGFP-expressed osteoblasts in green fluorescence, ALC staining region and merged image in opercula of Tg(Ola.Sp7:EGFP)^cy25 at 13 dpf. (O,P,Q) N1aICD-expressed osteoblasts in green fluorescence, ALC staining region and merged image in opercula of Tg(Ola.Sp7:EGFP)^cy25 and Tg(Ola.Sp7:N1aICD)^cy31 double transgenic fish at 13 dpf (averages ± SD; * p < 0.05; *** p < 0.005; n = fish number).

Expression Data

Gene:	GFP
Fish:	cy25Tg cy25Tg; cy31Tg
Anatomical Terms:	notochord osteoblast opercle osteoblast
Stage Range:	Days 14-20 to Days 21-29

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cy31Tg cy25Tg; cy31Tg
Observed In:	notochord bone mineralization opercle bone mineralization vertebral column bone mineralization
Stage Range:	Days 7-13 to Days 21-29

Phenotype Detail

Acknowledgments

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