FIGURE

Fig. 9

ID

ZDB-FIG-190724-21

Publication

Seda et al., 2019 - An FDA-Approved Drug Screen for Compounds Influencing Craniofacial Skeletal Development and Craniosynostosis

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Fig. 9

Functional assessment of calcineurin inhibition in skeletogenesis. A Relative expression of osteoblast differentiation marker genes normalised to untreated cells. * p < 0.05, **p < 0.005 (t test). B E18.5 calvarial explants cultured for 2 weeks in the presence or absence of 100 nMFK506. Top row: Low-power view of a control explant; note overlap of the midline parietal bones (arrows), showing continued osteogenesis of the sutures (1st and 2nd images). Whereas the DMSO-treated C342Y/+ coronal sutures undergo synostosis, FK506-treated C342Y/+ explants do not (arrows, 3rd and 4th images). The last image shows how the area of overlap of the frontal and parietal bones was measured over a fixed 1-mm length of suture (outlined) to gain a robust measure of suture width. These values are summarised over 33 explants, either wild-type (WT) or mutant (Mut), treated with DMSO or FK506. Values are given for both the left and right sutures for each explant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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