FIGURE

Fig. 6.

ID

ZDB-FIG-190723-882

Publication

Gjini et al., 2019 - Disruption of asxl1 results in myeloproliferative neoplasms in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6.

Combined loss of asxl1 and tet2 leads to MPN and AML in a subset of adult zebrafish. Morphological and quantitative analysis of blood cell types in the kidney marrow and peripheral blood of 5-month-old fish with the indicated genotypes. MGG staining was performed on kidney marrow smears. (A-H) Red arrows denote mature erythrocytes; light blue arrows denote myelomonocytes; green arrows denote lymphocytes; black arrows denote progenitor cells; and orange arrows denote immature red blood cells. (A) In wild-type fish, the kidney marrow hematopoietic cells showed normal maturation and morphology. (B) Five of 11 asxl1^+/⁻tet2^+/+ fish had an increased number of mature myeloid cells, indicating MPN; the remaining 6 fish had normal morphology. (C) Eight of 11 asxl1^+/⁻tet2^+/⁻ fish showed an increased number of mature myeloid cells, indicating MPN. (D) Two of 10 asxl1^+/⁻tet2^−/− fish showed increased myeloid blast cells, indicating AML. Four of the remaining 8 fish had MPN, while the other 4 had normal morphology. (E-H) Analysis of peripheral blood smears by MGG staining. (E) Wild-type fish had normal maturation and morphology of the blood cells. (F) Five of 11 asxl1^+/⁻tet2^+/+ fish showed rounded circulating immature red blood cells and increased mature myeloid cells. (G) Eight of 11 asxl1^+/⁻tet2^+/⁻ fish showed immature rounded circulating red blood cells. (H) Two of 10 asxl1^+/⁻tet2^−/− fish showed an increased number of myeloid blast cells, myelomonocytes and immature erythrocytes. (I-L) Forward- versus side-scatter analysis of kidney marrow cell populations in 5-month-old fish with the indicated genotypes. (M-P) Forward- versus side-scatter analysis of absolute cell numbers per liter of blood in 5-month-old fish with the indicated genotypes, showing increased myelomonocytes and decreased red blood cells in asxl1^+/⁻ fish. A subpopulation of asxl1^+/⁻tet2^−/− fish showed increased progenitor cell numbers in kidney marrow and blood. Mean values with s.e.m. are shown. Statistical analysis was done with Prism software. Unpaired Student's t-tests were performed in Prism to determine the P-value for each genotypic group compared with controls.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	asxl1^zdf34/+ asxl1^zdf34/+; tet2^zdf20/+ asxl1^zdf34/+; tet2^zdf20/zdf20
Observed In:	blood common myeloid progenitor blood erythroid lineage cell blood hematopoietic multipotent progenitor cell head kidney common myeloid progenitor head kidney erythroid lineage cell head kidney hematopoietic multipotent progenitor cell head kidney myeloid leukaemia
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.