Fig. 5

Activity of ventrally located V2a neurons in control and En1-DTA fish. a Lateral view of the spinal cord of Tg[chx10:GFP]. The horizontal dashed lines indicate the dorsal and ventral boundaries of the spinal cord. Ventrally located V2a neurons (the position from 0 to 0.5) are the subjects of the recordings (top panel). The bottom two panels show the images of a loose-patch recording. Scale bar, 20 μm. b An example of simultaneous recordings between ventrally located V2a neurons (loose-patch) and ventral root (VR) in control fish. c An example of simultaneous recordings between ventrally located V2a neurons (loose-patch) and VR in En1-DTA fish. d, e Numbers of spikes (d) and firing probability in each cycle (e) during fast/strong swim. Each circle represents each cell (n = 84 for ventrally located V2a neurons in control; n = 60 for ventrally located V2a neurons in En1-DTA). **P < 0.01 (Mann–Whitney U test, P = 1.3 × 10⁻¹⁰ [d], P = 3.7 × 10⁻¹⁴ [e]). Boxes represent the interquartile range (IQR) between first and third quartiles and the line inside represents the median. Whiskers denote the lowest and highest values within 1.5 × IQR from the first and third quartiles, respectively