Figure 6

Tracing of mossy fibers (MFs) using the RV method. The RV solution was injected into the left side of the cerebellum of adult Tg(cbln12:TVA-mCherry); Tg(cbln12:G) fish. The injected fish were then reared at 34–35.5°C for 10 days. The brains were harvested, fixed, and subjected to immunostaining. (A–K) Staining of brain cross sections with anti-mCherry (magenta) and anti-GFP (green) antibodies, and Hoechst (cyan). In the injected regions, GFP and TVA-mCherry were detected in neurons and neurites in the cerebellum (A,Aa). GFP but not TVA-mCherry was detected in neurons in the CPN (B), the paracommissural nucleus (PCN; C,D), the nucleus lateralis valvulae (NLV; E–G), the GC (H), the medial octavolateralis nucleus (MON; I), and the descending octaval nucleus (DON; J,K). Scale bar: (A) 200 μm, (B’) 50 μm. The magnification in (B–K) and (C’–K’) was the same as in (A) and (B’), respectively.