Figure 5

Selected retinal bipolar (BP) neurons that demonstrate the unusual range of morphologies observed at 13 and 17 days post-injury (DPI). Neurons were traced in Simple Neurite Tracer and then rendered in ImageJ's 3D viewer. (A,A') Neuron showing an apparently displaced soma and multiple neurites, shown from two different orientations (A = radial “side” view, A' = tangential “top-down” view). The cell body was only minimally traced due to limitations of SNT. (B,B') Neuron that has two distinct apparent dendritic trees connected to the same cell body. (C,C',D,D') Neurons with apparently simple morphologies sampled at 13 DPI and 17 DPI, respectively, each shown from two different orientations. In contrast to A and B, these BPs have a bipolar shape, but have smaller dendritic trees with fewer branches than most other neurons sampled at 13 and 17 DPI. The neuron in (C,C') appears to have more than one basal outgrowth (possibly multiple axons), all of them truncated, while the neuron in D has an axon with a normal appearance, but a single apical process rather than a dendritic tree. The cell body in (D,D') was only minimally traced due to limitations of SNT. Arrows designate investigator assignment of primary dendrites (green). Axons, red; incompletely traced cell bodies, grayscale. Scale bar(s) in all panels = 10 μm.