FIGURE

Fig. 1

ID

ZDB-FIG-190723-420

Publication

Aksoy et al., 2019 - Chemical reprogramming enhances homology-directed genome editing in zebrafish embryos

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Fig. 1

Overview of the in vivo homology-directed repair (HDR) detection system. a Schematic representation of the visual HDR readout. b The single guide RNA (sgRNA)-Cas9 complex targets the identical sequence in eGFP and eBFP2. Embryos are co-injected with HDR donor template to integrate the tdTomato transgene into the acta1-eBFP2 locus. c Confocal sections showing smyhc1:eGFP and acta1-eBFP2 expression and d merged images. Scale bars: 75 µm. e Cross-sectional representation of a zebrafish embryo showing slow and fast muscles. f Expression of smyhc1-eGFP in slow-muscle fibers (3 dpf). g Mosaic loss of smyhc1-eGFP expression in slow-muscle fibers (3 dpf) of embryos injected with a CRISPR (clustered regularly interspaced short palindromic repeat)/Cas9 complex targeting eGFP. Scale bars: 500 µm, main image and 100 µm, inset

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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