Figure 4

Kinetics of YB-1 aggregate formation. (a) confocal immunofluorescence of YB-1 (Green) in PAC2 cells at 26 °C (control) or after heat shock at 45 °C for the indicated times; (b) confocal immunofluorescence for YB-1(red) in PAC2 cells incubated at 45 °C for 45′ and then replaced at 26 °C for recovery from the heat shock at the indicated times; (c) percentage of cells forming YB-1 positive aggregates (plotted on the y-axes) incubated at 45 °C for the indicated times (white bars) and then allowed to recover for 15, 30 and 60 minutes at 26 °C (grey bars). Statistical analysis was performed using 1-way ANOVA and Dunnett’s multiple comparisons. Levels of significance are indicated (***p < 0.001) (see also Table S1); (d) quantification of YB-1 positive aggregate dimensions in PAC2 cells incubated at 45 °C for 45 minutes (white bar) and then allowed to recover at 26 °C for the indicated times (grey bars). Statistical analysis was performed using 1-way ANOVA and Dunnett’s multiple comparisons. Levels of significance are indicated (***p < 0.001) (see also Table S1).