FIGURE

Figure 3

ID

ZDB-FIG-190723-335

Publication

Kulkarni et al., 2018 - An In Vivo Zebrafish Model for Interrogating ROS-Mediated Pancreatic β-Cell Injury, Response, and Prevention.

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Figure 3

Metronidazole induces apoptosis signaling in β-cells. (a) Representative image of vehicle-treated zebrafish islets (n = 12) after fixing at 106 hpf. (b) Representative image of islets of zebrafish (NTR⁺) larvae (n = 12 per condition) treated with 2.5 mM or 7.5 mM MTZ at different time points and immune-stained for insulin and cleaved caspase 3 (Casp3^∗). (c) Quantification of Casp3^∗ intensity shows a significant increase after 6 hours of treatment in the β-cells of 7.5 mM MTZ-treated embryos compared to vehicle. (d) Representative immunofluorescence images of zebrafish (mpeg+) islets (N = 6/condition) treated with 7.5 mM MTZ showing macrophage invasion into islets (green arrows) and their engulfment of β-cells (yellow arrows). Data are presented as mean ± SEM (^∗p < 0.05). Statistical significance was determined by one-way ANOVA followed by post hoc Holm-Sidak test. Scale bar indicates 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Oxid Med Cell Longev