FIGURE

Figure 2

ID

ZDB-FIG-190723-303

Publication

Sharma et al., 2019 - Notch-mediated inhibition of neurogenesis is required for zebrafish spinal cord morphogenesis

Other Figures

All Figure Page

Back to All Figure Page

Figure 2

Notch pathway activity is required for spinal cord morphogenesis. (a,b) mib1 loss of function prevents DlA internalization (n = 9). (c,d) Combined inactivation of dld and dla disrupts apico-basal polarity in 9/10 embryos. (e,f) The γ-Secretase inhibitor LY411575 disrupts apico-basal polarity (n = 18). (g,h) Similarly the γ-Secretase inhibitor DAPT perturbs polarity in 4/5 embryos. (i-l) RNA injection of a constitutively activated form of Notch (NICD) restores neuro-epithelial morphology and apical aPKC localisation (k) but not DeltaD endocytosis (l) in 22/22 mib1 mutant embryos. (m,n) Polarity defects are observed in 20/46 embryos injected with RNA encoding dominant-negative Su(H) (DN-Su(H)). (o,p) RNA injection of constitutively activated Su(H) (CA-Su(H)) restores apico-basal polarity in 17/17 mib1 mutants. All images are dorsal views of the anterior spinal cord, anterior left. (a–f) 22 somites stage, (g,h,m,n) 30 somites stage, (i–l,o,p) 16 somites stage. Scalebars: 20 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.