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PP2A-Bδ controls myoblast fusion through regulation of <italic>ArgBP2</italic> expression.a RT-qPCR analysis of ArgBP2 mRNA levels in control (shCTL) and Bδ-KD (shBδ) C2C12 myoblasts during differentiation. Values are mean ± SD from three independent experiments. Two-way anova, with Bonferroni correction, ****P < 0.0001, **P < 0.01. b Fusion index of control (shCTL) and Bδ-knocked down (shBδ) C2C12 myoblasts transfected with a control siRNA or an siRNA against ArgBP2 (siArgBP2) from three independent experiments. One-way anova, with Tukey’s post-hoc test. **P < 0.01, ****P < 0.001. c–e Quantification of the curved trunk phenotype (c), d confocal imaging of F-actin (phalloidin, green, n = 10 for CTL Mo and n = 15 for Bδ Mo) and e quantification of the number of nuclei in fast skeletal myofibers of in 48-hpf zebrafish embryos injected with a control morpholino (CTL Mo, n = 5), or with a Bδ morpholino alone (Bδ Mo1, n = 3) or together with an ArgBP2 morpholino (Bδ Mo + ArgBP2 Mo, n = 10). Scale bars are 25 µm. One-way anova with Tuckey’s post-hoc test, **P < 0.01, *P < 0.05, ns: not significant
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