Figure 3

Tectal functional connectivity and neuronal assembly characteristics change over development. (A) Representation of the optic tectum as a graph. Each node (triangle) represents a neuron, and each edge represents correlation exceeding a threshold, i.e., functional connectivity between two neurons (gray lines for intra-PVL edges and orange lines for edges involving NP neurons). The solid black outline shows the boundary of the PVL. (B) Six communities (color coded) were detected in the graph presented in (A). (C) Functional connectivity metrics peaked at 5 dpf and declined afterwards. (D) Examples of the spatial organization of the spontaneous activity along the caudo-rostral axis of an intact (1–4) and enucleated larva (5–7) at different developmental stages. Scale bar represents 100 mm. Color bar: position along the caudo-rostral axis (the neurons are color coded according to the centroid azimuthal position of the assembly to which they belong; reproduced with permission from Avitan et al., 2017; Pietri et al., 2017).