Fig. 4

Adenine base editors modified by bpNLS enhance the base editing efficiency in zebrafish. a Schematic diagrams of three adenine base-editing system, zABE7.10, zABE7.10max, and zABE7.10-GE. The zABE7.10 system is a zebrafish codon-optimized version by IGE with SV40 NLS. The zABE7.10max is a version with bis-bpNLS and IGE codons. zABE7.10-GE system contain a GenScript-codon optimized sequence and bis-bpNLS. b The comparison of base editing efficiency of three versions of adenine base editor in zebrafish. c, f The sequencing results of the rps14, atp5b, wu:fc01d11, and musk gene in the zABE7.10max system. Target sequence (black), PAM region (blue), and the substituted bases (red) are indicated. The red arrows indicate the overlapped peaks. Values and error bars represent the mean of n = 3 biologically independent experiments. *p < 0.05