ZFIN ID: ZDB-FIG-190723-1719
Ouyang et al., 2019 - CPSF1 mutations are associated with early-onset high myopia and involved in retinal ganglion cell axon projection. Human molecular genetics   28(12):1959-1970 Full text @ Hum. Mol. Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Day 5

Figure 6

Retinal cells developed normally in WT larvae (A-D), std MO larvae (E-H) and cpsf1 morphants (I–L). (A, E, I) Co-labelling with anti-Pax6 (for ganglion and amacrine precursor cells, green) and anti-Zn5 (for mature RGCs and their axons, red) antibodies showed that the RGCs were mature. (B, F, J) Co-labelling with anti-HuC/D (for ganglion cells and amacrine cells, green) and anti-α PKC (for bipolar cells, red) antibodies indicated that the amacrine cells and bipolar cells developed normally. (C, G, K) Co-labelling with anti-GS (for Müller cells, green) and anti-GFAP (for glial cells and their axons, red) antibodies demonstrated that the Müller cells and glial cells had attained maturity. (D, H, L) Co-labelling with anti-Rho 1D4 (for long double-cone outer segments, green) and anti-Recoverin (for cone bipolar cells, red) antibodies demonstrated that the photoreceptors grew normally.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC AB control Day 5 amacrine cell
IHC Day 5 retinal ganglion cell
IHC AB standard conditions Day 5 amacrine cell
IHC Day 5 retinal ganglion cell
IHC AB + MO5-cpsf1 standard conditions Day 5 amacrine cell
IHC Day 5 retinal ganglion cell
Ab1-glul IHC AB control Day 5 Muller cell
IHC AB standard conditions Day 5 Muller cell
IHC AB + MO5-cpsf1 standard conditions Day 5 Muller cell
Ab1-pax6 IHC AB control Day 5 amacrine cell
IHC Day 5 retinal ganglion cell
IHC AB standard conditions Day 5 amacrine cell
IHC Day 5 retinal ganglion cell
IHC AB + MO5-cpsf1 standard conditions Day 5 amacrine cell
IHC Day 5 retinal ganglion cell
Ab1-prkca IHC AB control Day 5 retinal bipolar neuron
IHC AB standard conditions Day 5 retinal bipolar neuron
IHC AB + MO5-cpsf1 standard conditions Day 5 retinal bipolar neuron
Ab2-recoverin IHC AB control Day 5 cone retinal bipolar cell
IHC AB standard conditions Day 5 cone retinal bipolar cell
IHC AB + MO5-cpsf1 standard conditions Day 5 cone retinal bipolar cell
Ab3-rho IHC AB control Day 5 retinal cone cell cone photoreceptor outer segment
IHC AB standard conditions Day 5 retinal cone cell cone photoreceptor outer segment
IHC AB + MO5-cpsf1 standard conditions Day 5 retinal cone cell cone photoreceptor outer segment
Ab15-gfap IHC AB control Day 5 retina glial cell
IHC AB standard conditions Day 5 retina glial cell
IHC AB + MO5-cpsf1 standard conditions Day 5 retina glial cell
zn-5 IHC AB control Day 5 retinal ganglion cell
IHC Day 5 retinal ganglion cell axon
IHC AB standard conditions Day 5 retinal ganglion cell
IHC Day 5 retinal ganglion cell axon
IHC AB + MO5-cpsf1 standard conditions Day 5 retinal ganglion cell
IHC Day 5 retinal ganglion cell axon
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Human molecular genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Hum. Mol. Genet.