Retinal cells developed normally in WT larvae (A-D), std MO larvae (E-H) and cpsf1 morphants (I–L). (A, E, I) Co-labelling with anti-Pax6 (for ganglion and amacrine precursor cells, green) and anti-Zn5 (for mature RGCs and their axons, red) antibodies showed that the RGCs were mature. (B, F, J) Co-labelling with anti-HuC/D (for ganglion cells and amacrine cells, green) and anti-α PKC (for bipolar cells, red) antibodies indicated that the amacrine cells and bipolar cells developed normally. (C, G, K) Co-labelling with anti-GS (for Müller cells, green) and anti-GFAP (for glial cells and their axons, red) antibodies demonstrated that the Müller cells and glial cells had attained maturity. (D, H, L) Co-labelling with anti-Rho 1D4 (for long double-cone outer segments, green) and anti-Recoverin (for cone bipolar cells, red) antibodies demonstrated that the photoreceptors grew normally.