Comparison of Nrf family proteins. (A) The domain structures of four Nrf proteins in mammals were compared based on the six Neh domains of Nrf2. Striped color denotes the region that is partially conserved with the Neh4 domain. The ER binding domain (NHB1) that is specific to Nrf1 and -3 is also indicated; (B) Amino acid sequences of Nrf/Cnc transcription factors from mouse (Mm), chicken (Gg), clawed frog (Xt), zebrafish (Dr), ascidian (Ci), sea urchin (Sp), octopus (Ob), fruit fly (Dm), C. elegans (Ce) and Hydra (Hm). The amino acids identical to mouse Nrf2 are shaded in gray. Leucine residues comprising the zipper structure in Neh1, the DLG and ETGE motifs in Neh2, the VFLVPK motif in Neh3, the FxD/ExxxLLxE/D sequence in Neh4, the QxWxELxSxPELQ sequence in Neh5 and the DSGIS and DSAPGS motifs in Neh6 are shaded in yellow. Basic amino acid residues in the Neh1 basic region, lysine residues between the DLG and ETGE motif (ubiquitination sites) and serine/threonine residues (Ser-40, phosphorylation site) in the Neh2 domain are shown in pink, blue and orange letters, respectively.
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