Fig. 3

Slc41a1 knockdown evokes renal Mg²⁺ wasting in zebrafish. a–e Representative images of the normal morphologic phenotypes obtained in untreated (WT) 120 hpf zebrafish grown in Mg²⁺-free E3 medium and after injection of 16 ng control-MO/embryo or 4–16 ng slc41a1-MO/embryo (splice-site blocking MO). f Distribution of morphologic phenotypes in zebrafish larvae (120 hpf) untreated (WT) or after injection with different doses of slc41a1-MO (splice-site blocking MO) or control-MO. Fish were grown in Mg²⁺-free E3 medium. Numbers on top of the bars indicate the number of animals in each experimental condition. g, h Total Mg (g) and Ca (h) content in morphologically normal 120 hpf zebrafish grown in Mg²⁺-free E3 medium after injection with different doses of slc41a1-MO (splice-site blocking MO); the dose of zero represents injection with 16 ng control-MO/embryo (n = 10). i Distribution of morphologic phenotypes in zebrafish larvae (120 hpf) untreated (WT) or after injection with different doses of slc41a1-MO (splice-site blocking MO) or control-MO. Fish were grown in E3 medium (0.33 mmol/L Mg²⁺). Numbers on top of the bars indicate the number of animals in each experimental condition. j, k Total Mg (j) and Ca (k) content in morphologically normal 120 hpf zebrafish grown in E3 medium (0.33 mmol/L Mg²⁺) after injection with different doses of slc41a1-MO (splice-site blocking MO); the dose of zero represents injection with 16 ng control-MO/embryo (n = 10). g, h, j, k Data are presented as mean ± S.E.M. Asterisks indicate significant differences between control and experimental groups (*P < 0.05)