ZFIN ID: ZDB-FIG-190723-1453
Coronado et al., 2019 - Soybean Meal-Induced Intestinal Inflammation in Zebrafish Is T Cell-Dependent and Has a Th17 Cytokine Profile. Frontiers in immunology   10:610 Full text @ Front Immunol
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Days 7-13

Figure 1

Neutrophils, macrophages, and mast cells increase their presence in the gut during the inflammatory process triggered in the naïve feeding model. The number of neutrophils/macrophages (A), and mast cells (B) was quantified under different conditions (n, naïve; CTRL, control; INFL, inflammation) and time points (T0: 5 dpf; T4: 9 dpf). (C) Relative mRNA levels of gzma, nkla, and nkld were analyzed. Data were normalized against rpl13aand compared to T4 naïve condition (dotted line). (D)Scheme of a lateral view of a 9 dpf larva with the zone of the intestine used to quantify delimited by the red rectangle. (E–H) Lateral view of the intestine of a Tg(lysC:DsRed)xTg(mpeg1:Dendra2) larva showing fluorescently labeled neutrophils (red) and macrophages (green). (M–P) Lateral view of the intestine showing mast cells labeled by anti-tryptase immunohistochemistry. Zoom of macrophages and neutrophils (I–L) and mast cells (Q–T). *p < 0.05; ***p < 0.001; ****p < 0.0001. Scale bar 200μm. Experiments were done at least in three biological replicates with 20 individuals per condition.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Dendra2 uwm12Tg ; nz50Tg control Days 7-13 intestine macrophage IFL
uwm12Tg ; nz50Tg diet Days 7-13 intestine macrophage IFL
DsRed2 uwm12Tg ; nz50Tg control Days 7-13 intestine neutrophil IFL
uwm12Tg ; nz50Tg diet Days 7-13 intestine neutrophil IFL
gzma WT diet Days 7-13 intestine RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-TPSAB1 IHC WT control Days 7-13 intestine myeloid leukocyte
IHC WT diet Days 7-13 intestine myeloid leukocyte
Phenotype Details
Fish Conditions Stage Phenotype
WT diet Days 7-13 intestine gzma expression increased amount, abnormal
Days 7-13 intestine myeloid leukocyte increased amount, abnormal
uwm12Tg ; nz50Tg diet Days 7-13 intestine macrophage increased amount, abnormal
Days 7-13 intestine neutrophil increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Frontiers in immunology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front Immunol