ZFIN ID: ZDB-FIG-190723-1453
Coronado et al., 2019 - Soybean Meal-Induced Intestinal Inflammation in Zebrafish Is T Cell-Dependent and Has a Th17 Cytokine Profile. Frontiers in immunology   10:610 Full text @ Front Immunol
Anatomical Terms:
Stage: Days 7-13
Observed In:
Stage: Days 7-13

Figure 1

Neutrophils, macrophages, and mast cells increase their presence in the gut during the inflammatory process triggered in the naïve feeding model. The number of neutrophils/macrophages (A), and mast cells (B) was quantified under different conditions (n, naïve; CTRL, control; INFL, inflammation) and time points (T0: 5 dpf; T4: 9 dpf). (C) Relative mRNA levels of gzma, nkla, and nkld were analyzed. Data were normalized against rpl13aand compared to T4 naïve condition (dotted line). (D)Scheme of a lateral view of a 9 dpf larva with the zone of the intestine used to quantify delimited by the red rectangle. (E–H) Lateral view of the intestine of a Tg(lysC:DsRed)xTg(mpeg1:Dendra2) larva showing fluorescently labeled neutrophils (red) and macrophages (green). (M–P) Lateral view of the intestine showing mast cells labeled by anti-tryptase immunohistochemistry. Zoom of macrophages and neutrophils (I–L) and mast cells (Q–T). *p < 0.05; ***p < 0.001; ****p < 0.0001. Scale bar 200μm. Experiments were done at least in three biological replicates with 20 individuals per condition.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Dendra2 uwm12Tg ; nz50Tg control Days 7-13 intestine macrophage IFL
uwm12Tg ; nz50Tg diet Days 7-13 intestine macrophage IFL
DsRed2 uwm12Tg ; nz50Tg control Days 7-13 intestine neutrophil IFL
uwm12Tg ; nz50Tg diet Days 7-13 intestine neutrophil IFL
gzma WT diet Days 7-13 intestine RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-TPSAB1 IHC WT control Days 7-13 intestine myeloid leukocyte
IHC WT diet Days 7-13 intestine myeloid leukocyte
Phenotype Details
Fish Conditions Stage Phenotype
WT diet Days 7-13 intestine gzma expression increased amount, abnormal
Days 7-13 intestine myeloid leukocyte increased amount, abnormal
uwm12Tg ; nz50Tg diet Days 7-13 intestine macrophage increased amount, abnormal
Days 7-13 intestine neutrophil increased amount, abnormal
ZFIN wishes to thank the journal Frontiers in immunology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Front Immunol