FIGURE

Figure 2.

ID

ZDB-FIG-190723-1215

Publication

Dasgupta et al., 2018 - Mosaic Labeling and 3-Dimensional Morphological Analysis of Single Cells in the Zebrafish Left-right Organizer

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Figure 2.

Behavior and 2D morphology of DFC and KV cells during development.

Two transgenic zebrafish strains–Tg(dusp6:memGFP) and Tg(sox17:GFP-CAAX)–provide bright labeling of dorsal forerunner cells (DFCs) that give rise to Kupffer’s vesicle (KV) cells. A and B. Embryo diagrams (green represents DFC/KV cells) and inversed fluorescence images of membrane-localized GFP expression in DFCs in Tg(dusp6:memGFP) transgenic zebrafish at 80% epiboly stage (80% E) (A) and the tailbud stage (B) when migratory DFCs form a rosette structure. C and D. GFP expression in Tg(sox17:GFP-CAAX) transgenic zebrafish marks KV cell membranes during KV lumen formation at the 2 somite stage (2 ss) (C) and in the mature organ at 8 ss (D) after KV remodeling. A = Anterior; P = Posterior, L = Left; R = Right; D = Dorsal; V = Ventral. Anterior cells = blue, Posterior cells = red.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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