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RA does not regulate the somitic expression of Wnt16-Notch3 signaling pathway components.Shown are representative 17 hpf embryos following in situ hybridization analyses (A-J’). Lateral view (A-J) or dorsal view (A’-J’) of gene expression is shown with anterior oriented to the left. A’-J’ represent different views of the embryos shown in A-J. Compared to DMSO-treated controls (A, A’, C, C’, E, E’, G, G’), DEAB-treated embryos exhibit normal somitic expression levels of wnt16 (B, B’), and dlc (D, D’), mildly increased dld expression (F, F’), and increased notch3 somitic gene expression (H, H’). DEAB-treated embryos also exhibit normal expression levels of the Notch3 signaling pathway transcriptional target her9 (J, J’), when compared to DMSO-treated controls (I, I’). (K) Quantitative real-time PCR analysis of her9 expression in 17 hpf DMSO-treated controls and embryos treated with 5 μM DEAB. Shown is the relative quantity of her9 expression. Samples were normalized to ef1a and DMSO-treated was set to 1. Error bars indicate standard error of the mean. *Indicates the difference compared to control is significant by Student t test, P = 0.0198.
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