Fig. 4

LSN 2105786 treatment increased expression of Wnt targets and downstream genes in regenerating fin tissue. (A–F) In situ hybridization detecting lef1 transcript showed that the expression was restricted to the area in (arrowhead) and immediately surrounding (arrow) the blastema in DMSO treated fins, whereas, 5 nM LSN 2105786 treated fins showed an expansion of the expression domain as well as intensified staining at 1 and 2 dpa (A–D). Staining intensity at 3 dpa was not significantly altered in 5 nM LSN 2105786 treated fins compared to DMSO treated fins, but the expression area was expanded distally in treated fins (E,F). (O–R) In situ hybridization detecting shh transcript showed the expression in two distinct subsets of cells at the distal tip of the DMSO treated fins at 3 and 7 dpa (O,Q). Fins treated continuously with 5 nM LSN 2105786 showed expansion of the expression domain (P,R). (G–J) The expression of bmp2b was similar in the DMSO treated and 5 nM LSN 2105786 treated fins at 3 and 4 dpa. (K–N) In situ hybridization detecting bmp4transcript showed that the expression was dramatically increased in the 5 nM LSN 2105786 treated fin at 3 dpa (L) compared to DMSO treated fin (K). At 4 dpa, bmp4 was robustly expressed in the distal most epidermis of LSN 2105786 treated fin (N) but was not detected in the same region of DMSO treated fins (M). Scale bars in panels P and R are 3 cm. Bar in P applies for all panels, except Q and R. Bar in R applies to Q and R.