Fig. 3

Reduction of pcdh10a results in defects in melanoblast migration. (A-D) Lateral views of 72hpf embryos. Line represents approximate position of cross-section. (A) Wildtype embryos with normal larval pigment pattern and all four stripes present, with no internal pigment cells. (B) Schematic showing the difference in morphology and placement of internal pigment cells vs the other correctly patterned stripes (C) Embryos injected with 6 ng pcdh10aMorpholino containing several internal pigment cells. (D) pcdh10a -/- embryos also contain internal pigment cells. (A′-D′) cross-sectional views of a representational embryo from A-C. (E) AB embryos injected with 6 ng standard control Morpholino show no internal pigment cells. (F) ISH at 25hpf comparing wildtype embryo pcdh10a expression to pcdh10-/-mutant pcdh10a expression. (G) Quantification of the number of embryos with at least one internal pigment cell. (H) Quantification of the number of melanocytes at the lateral stripe on both sides of embryo. (I) Table, quantification of the ratio between number of internal melanocytes vs number of lateral stripe melanocytes. DS. Dorsal Stripe, LS. Lateral Stripe, VS. Ventral Strip, YS. Yolk Strip, NT, Neural Tube, n, Notochord, Y Yolk. Arrows show locations of example internal pigment cells. ns, Not Significant.