Fig. 7
- ID
- ZDB-FIG-190708-54
- Publication
- Kujawski et al., 2019 - penner/lgl2 is required for the integrity of the photoreceptor layer in the zebrafish retina
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Knockdown of Lgl2 in pen/lgl2 clutches leads to OLM and OPL abnormalities. Immunostaining (A–D′) and TEM (E–F′) of transverse retinal sections at 3 dpf of MO-injected pen/lgl2 clutches. (A–A″, C,C′) pen/lgl2 clutch injected with CNTR-MO and (B–B″,D,D′) pen/lgl2 clutch injected with ATG-MO1. ZO-1 (A,B), N-cadherin (A′,B′) and F-actin (C,D) localization to OLM (arrowheads) and OPL (arrows) is abnormal in morphants. Inset in B″ shows a projection of 15 optical planes in which discontinuity of the OLM is evident. Scale bars: 10?µm. (E,E′) pen/lgl2+/+ sibling injected with CNTR-MO shows a single, aligned layer of PRCs. E′ shows a higher magnification of a PRC adherens junction (arrowheads). (F,F′) pen/lgl2 mutant injected with ATG-MO1. PRCs have differentiated outer segments (green asterisks) but are disorganized. In the cells next to the disorganized area, normal adherens junctions are detected (F′, arrowheads). N, nucleus; M, mitochondria. Scale bar: 2?µm. |
| Antibody: | |
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| Fish: | |
| Knockdown Reagent: | |
| Anatomical Term: | |
| Stage: | Protruding-mouth |
| Fish: | |
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| Knockdown Reagent: | |
| Observed In: | |
| Stage: | Protruding-mouth |