Fig. 5

Details of islet1-GFP expression using fluorescent DAPI stain and choline acetyltransferase (ChAT) immunohistochemistry at 6 months of age. Conventions with lettering as in Figure 2. (A,A’) shows islet1-GFP positive axonal tract from the ventral nucleus of the ventral telencephalon (Vv) to the medial zone of the dorsal telencephalon (Dm). (B,B’) islet1-GFP axonal tract that projects from the intermediate hypothalamic nucleus (IN) to the dorsal thalamus. (C,D)Analysis of preoptic region at (C: DAPI, C’: islet1-GFP, C”: ChAT) level of magnocellular preoptic nucleus (PM) and (D: DAPI, D’: islet1-GFP, D”: ChAT) postoptic commissure (poc). Note that preoptic cholinergic cells are never double-labeled for islet1-GFP and strong cholinergic terminal field in the parvocellular superficial pretectal nucleus (PSp) originating in nucleus isthmi. Arrowhead in (C) indicates artifact (no DAPI cell nucleus seen). (E–E”) Magnification of midbrain tegmentum shows oculomotor nerve nucleus (NIII) and nerve exiting the brain (III). Asterisk points out hypothalamo-secondary gustatory nuclear tract. # indicates a ChAT positive fiber net (see Mueller et al., 2004). (F–F”)Region of secondary gustatory nucleus (SGN) shows ChAT cells in SGN and nucleus isthmi (NI) and superior reticular nucleus (SRN). Asterisk points out hypothalamo-secondary gustatory nuclear tract. # indicates a ChAT positive fiber net (see Mueller et al., 2004). (G–G”) Region of vagal sensory lobe (VaLo) and vagal motor nerve nucleus (NXm) shows some cholinergic vagal motor cells double-labeled for islet1-GFP. CCe, corpus cerebelli; Dm, medial zone of dorsal telencephalon; Hd/Hv, dorsal/ventral zone of periventricular hypothalamus; IN, intermediate hypothalamic nucleus; NI, nucleus isthmi; NIII, oculomotor nerve nucleus; NXm, vagal motor nerve nucleus; ot, optic tract; PM, magnocellular preoptic nucleus; poc, postoptic commissure; PPp, posterior parvocellular preoptic nucleus; PSp, parvocellular superficial pretectal nucleus; PTh, prethalamus; SC, suprachiasmatic nucleus; SGN, secondary gustatory nucleus; SRF, superior reticular formation; SRN, superior reticular nucleus; VaLo, vagal (sensory) lobe; Vdd/Vdv, dorsal/ventral subnucleus of dorsal nucleus of ventral telencephalon; Vv, ventral nucleus of the ventral telencephalon; III, oculomotor nerve; IV, trochlear nerve.