FIGURE

Fig. 5

ID

ZDB-FIG-190627-64

Publication

Zhang et al., 2019 - In Vitro and In Vivo Anti-Inflammatory Effects of Polyphyllin VII through Downregulating MAPK and NF-κB Pathways

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Fig. 5

Anti-inflammatory and protective effects of PP7 in LPS-induced inflammation in zebrafish embryo. Zebrafish embryos at 8 h post-fertilization (hpf) were pretreated with PP7 and exposed to LPS. (A) The NO generation in zebrafish larvae was measured by image analysis and fluorescence microscope after staining with 4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate (DAF-FM-DA). Scale bars represent 200 μm. (B) Quantitative analysis of fluorescence intensity of (A) using an image J program. Effects of PP7 on heart beat rate (C) and yolk sac edema size (D) of LPS treated zebrafish embryos. Data are represented as means ± SD from three independent experiments. n = 10/group, * p < 0.05, ** p < 0.01 as compared with LPS-treated alone.

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