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Fig. 1

ID

ZDB-FIG-190625-39

Publication

Hadzhiev et al., 2019 - A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation

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Fig. 1

MOVIE detects native transcripts at the site of transcription in living zebrafish embryos. a Left: schematic of the detection principle of transcript imaging by MOVIE at the miR430 primary miRNA gene cluster. Right: Image of whole embryo, imaged in vivo by lightsheet microscopy, injected with three antisense, FITC-labelled morpholinos (MO) targeting miR430 priRNA (green) and mRuby3:H2B fusion protein as nuclear marker (red). Arrows point at signal accumulation, appearing as double green punctae within the nuclei, labelled by mRuby3:H2B (arrowheads). bRepresentative images of live embryos, imaged in vivo at high/oblong by lightsheet microscopy, injected at zygote stage with triple and single sense, antisense and mismatch-containing, FITC-labelled morpholinos and transcription blockers (α-amanitin or triptolide, n = 6 embryos each). c Transcript signal detection before and after 1-hour triptolide treatment started at 256-cell stage (0 h), (n = 6 embryos each). Stages of embryos as indicated in panels. trip triptolide, as antisense, s sense, MO morpholino oligonucleotide (where number depicts specific MO, for details see Supplementary Table 1), 5 MM 5 mismatch containing, h hours after treatment. Scale bars: a 50 µm b, c 10 µm

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.