FIGURE

Fig. 1-S1

ID

ZDB-FIG-190625-12

Publication

Yamaguchi et al., 2019 - zGrad is a nanobody-based degron system that inactivates proteins in zebrafish

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Fig. 1-S1

Characterization of the AID system in zebrafish.

(A) Images of embryos injected with sfGFP-mAID mRNA and different amounts of OsTIR1-mCherrymRNA and incubated with 0.2%EtOH (left) or the auxin IAA 500 µM (right) over a 3 hr time course. Scale bar: 1 mm. (B) Images of embryos injected with zGrad mRNA and OsTIR1-mCherry mRNA at 24 hpf. Scale bar: 1 mm. (C) Percentage of deformed embryos among embryos injected with zGrad mRNA and OsTIR1-mCherry mRNA at 24 hpf.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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