Fig. 4
- ID
- ZDB-FIG-190624-17
- Publication
- Obermann et al., 2019 - The Surface Proteome of Adult Neural Stem Cells in Zebrafish Unveils Long-Range Cell-Cell Connections and Age-Related Changes in Responsiveness to IGF
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The IGF Signaling Pathway Declines with Age (A) Hierarchical clustering of the IGF, Insulin, and Akt pathway protein members identified in the surface fraction, revealing the differential expressions in young and old dorsal surface fractions (scale: log2 young/old). Insulin receptor B, as well as IGF1 receptors a and b, are downregulated with age, while some other components are upregulated (i.e., MAP2K1 and BRAF). (B) Representation of the signaling pathway with color coding (blue, upregulated young; yellow, upregulated old). (C–J) Phosphorylated IGF1R (P-IGF1R) immunohistochemistry (Tyr1161) on young (5 months, C–F) and old (2 years, G–J) brains as a close-up view on the ventricular surface of the dorsal pallium depicted as single confocal planes. The radial glia somata, located at the border of the ventricle, express P-IGF1R (yellow arrows). We observe the distinct expression levels of P-IGF1R in young and old brains in a total of 4 young and 4 old immunosamples. Scale bar, 10 μm |