Fig. 7

Dexamethasone mediated immunosuppression does not alter the survival of itln3 deficient zebrafish in a mycobacterial infection. (A) A schematic representation of the performed experiment. M. marinum inoculate used in the infections were 47 CFU (SD 4 CFU). (B) Representative flow cytometry plots in WT (itln3^uta148) zebrafish at -1 wpi and 2 wpi used for quantifying lymphocyte, myeloid cell and precursor cell populations. FSC = forward scatter, SSC = side scatter. (C,D) Lymphocyte fractions of the total cell populations for both WT and itln3 knockout zebrafish at -1 wpi and 2 wpi. Both the itln3^uta145 (n = 12 in all groups) and itln3^uta148 background fish (n = 8 in all groups) are shown. Blood cell samples were run as technical duplicates. (E,F) M. marinum burden (log10) in 100 ng of zebrafish DNA were measured at 2 wpi and 4 wpi by qPCR in the infected dexamethasone treated zebrafish organ blocks (without the kidney). All bacterial quantification samples were run once. Group sizes at 2 and 4 wpi, respectively, were as follows: WT (itln3^uta145) n = 9, n = 8; iltn3^{uta145/uta145}n = 9, n = 10; WT (itln3^uta148) n = 6, n = 9 and iltn3^{uta148/uta148} n = 7, n = 8. A two-tailed Mann-Whitney test was used in the statistical comparison of differences.