Knockdown with morpholinos targeted to miR‐18a, miR‐18b, or miR‐18c results in more differentiated cone photoreceptors. (A) ZPR‐1 (cone) immunolabeling in 70 hpf larvae that were injected at the single‐cell stage with standard control, miR‐18a, miR‐18b, or miR‐18c morpholinos; note that, due to sequence similarities, each morpholino might comprehensively knock down miR‐18a, b, and c. (B) In situ hybridization for miR‐18a, comparing expression in larvae injected with standard control morpholino (left) with miR‐18a knockdown (right). (C) Cone photoreceptor counts presented as the mean of one eye per fish (n = 3) counted in the centermost cross section in the vicinity of the optic nerve. (D) Total number of BrdU‐labeled cells presented as the mean of one eye per fish (n = 3) counted in the centermost cross section in the vicinity of the optic nerve. Error bars show standard deviation and counts were statistically compared using a Student’s ttest. [Colour figure can be viewed at wileyonlinelibrary.com]
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