FIGURE

Fig. 5

ID

ZDB-FIG-190606-48

Publication

Goudarzi et al., 2019 - Individual long non-coding RNAs have no overt functions in zebrafish embryogenesis, viability and fertility

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Fig. 5

No non-coding function for squint 3’UTR.

(A) The position of untranslated regions (brown), coding region (green), putative Dorsal Localization Element- DLE (blue) and the gene deletion (red dashed line) in the squint genomic locus. Arrows flanking black dotted line mark the primer binding sites for qRT-PCR product. (B) In situ hybridization for squint at 8-cell stage on wild-type (18/20) and MZsquint^a175(17/17) embryos. (C) qRT-PCR for squint and eif4ebp1 on wild-type and MZsquint^a175 embryos at 1-cell stage. (D) Two representative MZsquint^a175 embryos. (E) MZsquint^a175 embryonic phenotype (N = 4 independent crosses, n = 360 embryos). The statistical significance of the observed changes was determined using t-test analysis and represented by star marks (ns, *, **, ***, and **** respectively mark p-values≥0.05,<0.05,<0.01,<0.001 and<0.0001).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ndr1^a175/a175 (AB/TL)
Observed In:	eye
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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