Fig. 6
The nucleus in the micropylar cell is composed of two closely juxtaposed nuclei. (A-C’”) Single confocal plane of immunofluorescence of Taz and β-Catenin in whole mount wild type oocytes at three stages: late II/ early III (A-A’”), mid III (B-B’”) and late III (C-C’”) shows β-Catenin and DAPI in the cell membrane and nucleus respectively. The nucleus in most micropylar cells (57/60) is composed of two closely juxtaposed nuclei. (D-F’”) Similarly, single confocal planes of immunofluorescence of Taz and Nup107 (nuclear membrane marker) in stage late II/ early III (D-D’”), mid III (E-E’”) and late III (F-F’”) oocytes show two closely juxtaposed nuclei surrounded by continuous nuclear membranes in the micropylar cell (n = 32 oocytes). Scale bar, 10 μm. |