FIGURE

Fig. 2

ID

ZDB-FIG-190307-6

Publication

Treichel et al., 2018 - Development of an Embryonic Zebrafish Oligodendrocyte-Neuron Mixed Coculture System

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Fig. 2

Tg(myrf:EGFP) is an oligodendrocyte-lineage-specific reporter in the zebrafish spinal cord. (A) Tg(myrf:EGFP) reporter expression at 75 hpf shows EGFP+ cells distributed in the ventral and dorsal spinal cord. Heart EGFP expression results from the myl7:EGFP transgenesis marker. High magnification confocal images of (B) Tg(myrf:EGFP)/Tg(sox10:mRFP) and (C) Tg(myrf:EGFP)/Tg(olig2:DsRed) double transgenic embryos at 48 hpf. Scale bars = 500 μm (A) and 5 μm (B, C). (D) Plot of overlap between Tg(myrf:EGFP) and established OPC reporters. Nsox10 = 11 fish, nsox10 = 108 cells; Nolig2 = 13 fish, nolig2 = 88 cells. Bars show mean ± SEM. For (B, C) imaging performed at somite 6.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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