FIGURE

Fig. 7

ID

ZDB-FIG-190301-5

Publication

Ramanagoudr-Bhojappa et al., 2018 - Multiplexed CRISPR/Cas9-mediated knockout of 19 Fanconi anemia pathway genes in zebrafish revealed their roles in growth, sexual development and fertility

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Fig. 7

Homozygous knockout fish are fertile except for fancd1 and fancj knockout males.

(A) Viability of embryos from outbred homozygous knockout fish at 24 hpf was determined to assess their fertility. Except fancd1 and fancj knockout males, all other knockouts (both males and females) had no fertility defect. For fancd1^hg45/hg45 a small fraction of embryos (<5%) were found viable at 24 hpf. (B-C) Histology images (20x objective) of hematoxylin and eosin stained adult testis of fancd1^hg45/hg45 (B) and fancj^hg56/hg56 fish (C). The red arrows point to the area where mature spermatozoa reside in the testis. Empty intra-testicular ducts were observed in homozygous mutant testis (ii) but not in the heterozygous testis (i).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	brca2^hg45/hg45 brip1^hg56/hg56
Observed In:	male organism testis sperm whole organism
Stage:	Adult

Phenotype Detail

Acknowledgments

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