Fig. 1

Two functional paralogues of mia40 are present in the zebrafish genome.

(A) Schematics of the protein products of the two paralogues of mia40 in zebrafish. Disulfide bonds between conserved cysteines are marked. (B) The two zebrafish paralogues were expressed in yeast strains devoid of MIA40. The lethal phenotype of yeast strains depleted of MIA40 is rescued by the expression of mia40a or mia40b. Growth is restored to levels of native yeast Mia40 upon fusion of Danio rerio paralogues to the b2-mitochondria targeting presequence. (C-E) The spatiotemporal expression of mia40 paralogues in zebrafish development was monitored by RT-PCR (C), RT-qPCR (D) and in situ hybridization (E). (C) Transcripts for both mia40 paralogues are maternally contributed as well as expressed by the embryo during early zebrafish development. (D) Relative to rpl13a expression, mia40a mRNA levels are significantly higher compared to the levels of mia40b. Error bars correspond to SEM; C_T values are presented in S8 Table. (E) mRNA expression patterns of mia40a and mia40b by in situ hybridization at indicated time points. At 24 hpf, mia40a expression is detected in the eye, brain (arrow), and somites, while mia40b expression is detected only in the head. Insets show a higher magnification of the somites. At 3 dpf, mia40a expression is enriched in the eye (white arrowhead) and brain (black arrows), and both mia40a and mia40b are expressed in the liver (black arrowheads). mia40a expression is greatly reduced by 5 dpf, whereas mia40b expression is strong in the head, branchial arches, epidermis, and lateral line primordia.