Fig. 2

Fgf3 facilitates proliferation and neurogenesis of Islet1 motor neurons after spinal cord injury. a, b Three days post spinal cord injury (dpi), very few newly generated BrdU+ (red) cells express Islet1+ (green) motor neuron marker (a), unless treated with Fgf3 for three days (B, arrowhead). White box indicates region shown at higher magnification with individual and merged channels (A? - A?? and B? ? B??). (C ? F) Analysis of controls at 10 dpi shows that usually only a small proportion of newly generated BrdU+ cells usually become Islet1+ motor neurons (c, f). However, treatment with Fgf3 for three days facilitates both overall proliferation (increased number of BrdU+ cells) and specifically the proportion of newly generated cells that are becoming Islet1+ motor neurons (e, f), while overall Islet+ numbers, but not the newly generated BrdU+ cohort is significantly reduced when Fgf3 signalling is inhibited (d, f). Results in C show mean ± SEM, (n?=?5 fish /group) *** p?<?0.001; N.S.: not significant. Scale bar in B (for A and B) is 50??m, scale bar in B?? (for A? ? A?? and B? ? B??) is 10??m and scale bar in F? (for D - F is 100??m)