Fig. 3

Segmental analysis of eTw-5 and eTw-11 enhancers in zebrafish embryos at 72 hpf.

(A) eTw-5 was divided into three overlapping segments: Segment 1, segment 2 and segment 3. The UCSC genome browser (http://genome.ucsc.edu) conservation track shows that segment 2 is most conserved between humans and fish. (B) A graph displaying the number of embryos presenting GFP expression in the pectoral fin and branchial arch tissues from all live embryos at 72 hpf. (C) Zebrafish enhancer assay results for eTw-5 segments. The full sequence of the p300 ChIP-seq peak drove GFP expression in the pectoral and caudal fins, branchial arch and somitic muscles. Segment 1 did not drive GFP expression, segment 2 drove GFP expression in the fins and branchial arch and segment 3 drove GFP expression mostly in somitic muscles and to some extent in the branchial arch. (D) eTw-11 was divided into four overlapping segments (segments 1–4). The UCSC genome browser (http://genome.ucsc.edu) conservation track shows that segment 3, 4 are conserved from zebrafish to humans. (E) A graph displaying the number of embryos with GFP expression in the pectoral fin and branchial arch tissues from all live embryos at 72 hpf. (F) Zebrafish enhancer assay results for eTw-11 segments: the full sequence of the p300 ChIP-seq peak drove GFP expression in the base of the pectoral fin, in the otic vesicle and branchial arch. Segments 1, 3 and 4 did not drive GFP expression, while segment 2 drove dominant GFP expression in the base of the pectoral fin and the branchial arch.