Fig. S2

4.1Ba MO does not alter structures involved in the sensorimotor circuit.

(A) Lateral view of CaPs, the myotome, and the NMJ in the trunk of 26 hpf Ctl MO and 4.1Ba MO injected embryos. (B—D) No differences were found in the number of branch points and terminal points (B), number of processes per cell (C), or the length of the processes (D). (E) Quantification of acetylcholine puncta at the neuromuscular junction. There were no changes in the number of acetylcholine puncta at the NMJ. (F) Failure rate of touch-evoked responses represented as a percentage of stimuli. No effect was seen in the ability of the embryos to respond to a tactile stimulus. (G) Frequency of spontaneous coils represented as the number of spontaneous coils per minute at 19 hpf. (H) Kinematic analysis of individual C-tail bends at 19 hpf. No differences were seen in the frequency or velocity of spontaneous coils. Error bars: s.e.m. Scale bar in A: 10 μm—CaPs; 50 μm—muscle and NMJ.