FIGURE

Fig. 1-S1

ID

ZDB-FIG-181003-6

Publication

Ignatius et al., 2018 - tp53 deficiency causes a wide tumor spectrum and increases embryonal rhabdomyosarcoma metastasis in zebrafish.

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Fig. 1-S1

tp53^del/del zebrafish survive at expected Mendelian ratios, lack Tp53 protein expression, and are resistant to irradiation-induced cell death.

(A) Survival of animals by genotype from a heterozygous tp53^wt/del in-cross. tp53 homozygous wild-type (wt/wt), heterozygous (wt/del), and homozygous (del/del) mutant fish. (B) Western blot analysis at 24 hr post-fertilization (hpf) whole embryos. Actin is used as a loading control. (C–D) TUNEL staining performed on tp53^wt/wt and tp53^del/del embryos following gamma-irradiation at 24hpf (16 Gray) and fixation at 30hpf. Whole embryos images are shown for representative animals of each genotype (C). Quantification of TUNEL-positive cells within 1000 micron² area. Regions where cells were counted are outlined by the white boxes in panel C. p<0.001 by Student’s T-test.

Expression Data

Antibody:	Ab11-tp53
Fish:	tp53^fb105/fb105
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT tp53^fb105/fb105
Condition:	gamma ray
Observed In:	apoptotic process whole organism
Stage Range:	Prim-5 to Prim-15

Phenotype Detail

Acknowledgments

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