Fig. 4

M. marinum causes damage to blood vessels and surrounding tissue. (a) Electron microscopy and (b) confocal imaging merged into (c) correlative light and electron microscopy of 9 dpf Tg(kdrl:mCherry)^is5 larva with red fluorescent blood vessels infected with green fluorescent M. marinum E11, after phagocyte depletion. Nuclei were stained with DAPI after fixation (cyan). Boxed areas are enlarged in (d)–(g). Scale bar in c = 2 μm. (d) High magnification of infected blood vessel in brain of infected zebrafish with erythrocyte, vessel lumen, and intact basal lamina on one side of the blood vessel visible. Scale bar = 1 μm. (e) Intact basal lamina at uninfected part of this infected blood vessel. Scale bar = 500 nm. (f) As a consequence of bacterial replication and invasion of endothelial cells, the basal lamina is disrupted (red dotted line). Scale bar = 1 μm. (g,g') higher magnification of the disrupted basal lamina. Scale bar = 500 nm. Er = Erythrocyte, BL = blood vessel lumen, M = M. marinum, N = nucleus