Fig. 8

Teratogenic heat resilient developmental windows can be used for production of gynogenic diploids. A?C: Metaphase chromosome spreads show that diploids have 50 (24 hpf, A), gynogenic haploids have 25 (48 hpf, B), and heat shocked gynogenic haploids have 50 (48 hpf, C) chromosome counts, indicating a genome doubling event in the haploids upon transient heat shock. D?F: Live images at six dpf of diploids (D), haploids (E), and gynogenic diploids (F) show swim bladder inflation in diploids (D) and gynogenic diploids (F), but not in haploids (E). G?I: IVF using spermatozoa from Tg(cmlc2:mCherry) males result in transmission of the transgene from the male genome into the progeny. J?L: IVF using UV irradiated spermatozoa from Tg(cmlc2:mCherry) males produces haploids, which after a heat shock become gynogenic diploids with only the maternal nontransgene carrying genome. G,J: brightfield, H,K: cardiac mCherry fluorescence channel, I,L: merge. M: Quantification of swim bladder inflation in multiple trials of gynogenic diploids that were generated by transient heat shock of haploids at 12, 18, and 24 mpf.