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ZIRC
ZFIN ID: ZDB-FIG-180828-32
Huo et al., 2018 - Prednisolone induces osteoporosis-like phenotypes via focal adhesion signaling pathway in zebrafish larvae. Biology Open   7(7) Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Days 7-13
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Days 7-13

Fig. 5

Osteoporosis-like phenotypes caused by prednisolone through itga10 and itgbl1. (A) qRT-PCR confirmed that itga10 expression is down-regulated by prednisolone. (B) qRT-PCR confirmed that itgbl1 expression is down-regulated by prednisolone. (C) The gRNAs for itga10 and itgbl1 were designed and injected into single-cell embryos. Whole mount skeletal staining was performed on fixed tissues. (D,E) Digital image analysis of stained area, and staining density by fluorescence intensity measurement. The stained mineralized tissue was quantified using analysis software. Mean values are plotted (n=5) and the Student’s t-test was performed to determine statistical significance.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
itga10 WT chemical treatment by environment: prednisolone Days 7-13 whole organism RTPCR
itgbl1 WT chemical treatment by environment: prednisolone Days 7-13 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: prednisolone Days 7-13 whole organism itga10 expression decreased amount, abnormal
Days 7-13 whole organism itgbl1 expression decreased amount, abnormal
WT + CRISPR1-itga10 standard conditions Days 7-13 skeletal tissue decreased area, abnormal
WT + CRISPR1-itgbl1 standard conditions Days 7-13 skeletal tissue decreased area, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open