Fig. S8
- ID
- ZDB-FIG-180822-29
- Publication
- Pinto et al., 2018 - Identification of Dmrt2a downstream genes during zebrafish early development using a timely controlled approach
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dmrt2a-MO is specific for the validated genes. A1-F3? In situ hybridisation results depicting the six genes validated in this work. A1-A3? etv2 expression pattern in wildtype embryos (A1-A1?), in dmrt2a ?100?/? ;dmrt2b ?/? embryos injected with ctrMO (A2-A2?) and dmrt2a-MO (A3-A3?). B1-B3?? foxj1b expression pattern in wildtype embryos (B1-B1??), in dmrt2a ?100?/? ;dmrt2b ?/? embryos injected with ctrMO (B2-B2??) and dmrt2a-MO (B3-B3??). C1-C3? cyp1a expression pattern in wildtype embryos (C1, C1?), in dmrt2a ?100?/? ;dmrt2b ?/? embryos injected with ctrMO (C2, C2?) and dmrt2a-MO (C3, C3?). D1-D3 cxcl12b expression pattern in wildtype embryos (D1), in dmrt2a ?100?/? ;dmrt2b ?/? embryos injected with ctrMO (D2) and dmrt2a-MO (D3). E1-E3 pxdc1b expression pattern in wildtype embryos (E1), in dmrt2a ?100?/? ;dmrt2b ?/? embryos injected with ctrMO (E2) and dmrt2a-MO (E3). F1-F3? foxc1b expression pattern in wildtype embryos (F1, F1?), in dmrt2a ?100?/? ;dmrt2b ?/? embryos injected with ctrMO (F2, F2?) and dmrt2a-MO (F3, F3?). We did not observe obvious differences between the three different conditions evaluated. All embryos were collected between 3 and 4-somite stage. (A1-A3, B1-B3, C1-C3, D1-D3, E1-E3, F1-F3) Lateral view, anterior to the left. (A1?-A3?, B1?-B3?) Dorsal-anterior view, anterior to the top. (A1?-A3?, B1??-B3??, C1?-C3?, F1?-F3?) Dorsal-posterior view, anterior to the top. (B1?-B3?) Dorsal-medial view, anterior to the top. ctrMO: control morpholino, WT: wildtype. (TIF 8561 kb) |