FIGURE

Fig. 5

ID

ZDB-FIG-180816-5

Publication

Davis et al., 2018 - ETS transcription factor Etsrp / Etv2 is required for lymphangiogenesis and directly regulates vegfr3 / flt4 expression

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Fig. 5

Etv2 depletion by caged MO photoactivation results in reduction of prox1a:GFP⁺ lymphatic progenitor cells. TgBAC(prox1a:KalT4-UAS:uncTagRFP); Tg(UAS:GFP) live embryos were imaged by confocal microscopy at 32 hpf. The number of prox1a:GFP⁺ cells is significantly reduced, 1.8 ± 0.32 cells, in embryos where etv2 is depleted at 24 hpf by caged MO photoactivation (n = 12) (B), compared to uninjected embryos (A) (5.1 ± 0.38 cells, n = 13, p < 0.0001, two tailed student's t-test). Arrows indicate prox1a:GFP⁺ endothelial cells. (C) Quantification of prox1a:GFP positive cells across the width of 9 somites in the trunk region. Mean±SEM is shown. Individual data points are shown in Fig. S4B. This experiment has been replicated twice.

Expression Data

Gene:	EGFP
Fish:	nim5Tg; nkuasgfp1aTg nim5Tg; nkuasgfp1aTg + MO2-etsrp
Knockdown Reagent:	MO2-etsrp
Anatomical Term:	vascular lymphangioblast
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nim5Tg; nkuasgfp1aTg + MO2-etsrp
Knockdown Reagent:	MO2-etsrp
Observed In:	vascular lymphangioblast
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 440(1), Davis, J.A., Koenig, A.L., Lubert, A., Chestnut, B., Liu, F., Desai, S.P., Winkler, T., Pociute, K., Choi, K., Sumanas, S., ETS transcription factor Etsrp / Etv2 is required for lymphangiogenesis and directly regulates vegfr3 / flt4 expression, 40-52, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.