FIGURE

Fig. 3

ID

ZDB-FIG-180712-81

Publication

Kelu et al., 2018 - TPC2-mediated Ca²⁺ signaling is required for the establishment of synchronized activity in developing zebrafish primary motor neurons.

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Fig. 3

Effect of depleting the acidic Ca²⁺ stores and antagonizing TPCs on the spontaneous Ca²⁺ activity of the CaPs in SAIGFF213A;UAS:GCaMP7a embryos at ~18 hpf and ~24 hpf. Embryos were (A, B) untreated, or (C-H) they were treated with: (C,D) 1% DMSO, (E,F) 1 µM bafilomycin A1, or (G, H) 250 µM trans-ned-19. Drug treatments started at ~ 17 hpf, and finished at either (A,C,E,G) ~ 18 hpf or (B,D,F,H) ~ 24 hpf. (Ai-Hiv) Time-lapse fluorescence images, and (Av-Hv) line graphs as described in Fig. 1.

Expression Data

Gene:	GCaMP
Fish:	nksaigff213aGt; zf415Tg
Conditions:	chemical treatment by environment: bafilomycin A1 chemical treatment by environment: trans-Ned 19
Anatomical Term:	CaP motoneuron
Stage Range:	14-19 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nksaigff213aGt; zf415Tg
Conditions:	chemical treatment by environment: bafilomycin A1 chemical treatment by environment: trans-Ned 19
Observed In:	CaP motoneuron release of sequestered calcium ion into cytosol
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 438(1), Kelu, J.J., Webb, S.E., Galione, A., Miller, A.L., TPC2-mediated Ca²⁺ signaling is required for the establishment of synchronized activity in developing zebrafish primary motor neurons., 57-68, Copyright (2018) with permission from Elsevier. Full text @ Dev. Biol.