FIGURE

Fig. S3

ID

ZDB-FIG-180712-33

Publication

Sánchez-Iranzo et al., 2018 - Transient fibrosis resolves via fibroblast inactivation in the regenerating zebrafish heart

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Fig. S3

Kidney marrow-derived cells do not contribute to collagen deposition during fibrotic response to cryoinjury. (A) Kidney marrow-derived cells from a transgenic line ubiquitously expressing GFP and mCherry under the control of col1a2 regulatory regions were transplanted into irradiated wild-type adult zebrafish. After kidney marrow reconstitution, host hearts were cryoinjured. Expression of mCherry and GFP was assessed at 7 dpi by immunofluorescence of sections. MHC marks the myocardium, and nuclei are counterstained with DAPI. (B) Whole-heart section of a transplanted heart at 7 dpi. (C–E) Merged and single channels of the boxed area in B. No mCherry signal is visible. (F) Control whole-heart section from a col1a2:mCherry transgenic fish at 7 dpi. (G–I) Merged and single channels of the boxed section shown in F; mCherry signal is detected in the epicardium and injury areas. Asterisks indicate the injury area. [Scale bars, 25 μm (C and G) and 100 μm (B and F).]

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA