Fig. 1

Exposure to iAs is toxic to zebrafish embryos. (A) Survival analysis of zebrafish treated with iAs from 4?hpf through 6 dpf. Fish were scored daily for mortality (n?2 clutches, >80 embryos per condition, Table S1). Red arrow indicates addition of iAs. Concentration of iAs used throughout the manuscript is shown in red and indicated by an asterisk. (B) Bright-field images of representative wild-type control (top) and arsenic-exposed (bottom) 5?dpf zebrafish larvae. Arrows indicate arsenic-induced phenotypes, including shortened body length, edema, clustering of pigment cells, under-consumption of yolk, and a small head. (C) Exposure to increasing doses of iAs from 4?hpf to 5?dpf led to an accumulation of phenotypes. The proportion of surviving embryos at 5?dpf that were affected increased with increasing concentrations of iAs (n=2 clutches exposed to 0.1?mM or 0.3?mM, n=3 clutches exposed to 0.6?mM, 1.0?mM or 1.4?mM, >30 fish exposed per treatment condition, Table S1). hpf, hours post-fertilization; dpf, days post-fertilization; iAs, inorganic arsenic.