Fig. S5

Overexpression of oep results in expansion of bhik expression domain towards the animal pole.

A) Lateral view of the expression of bhik in individual 60% epiboly embryos injected with egfp or oep mRNA. B) Signal intensity of the lateral area averaged over multiple embryos is shown in the heatmap panels. Average intensity of bhik staining profiles along the animal-vegetal axis for both treatments are shown in heatmap bars. Corresponding values are plotted in the graph. Colored envelopes of the curves represent the standard error of the mean, based on 12 (oep) and 13 (egfp) embryos. bhik expression is similar in oep- and egfp-injected most animally and vegetally, but in oep-injected embryos, the average intensity is higher in the mesendoderm area, and the expression domain is extended towards the animal pole. Shown is a representative example of three biological replicates. Although this expansion of the reach of Nodal signaling does not impede development ((Zhang et al., 1998), this study), the decrease in oep expression might be relevant under altered environmental conditions.