FIGURE

Fig. 5

ID
ZDB-FIG-180621-55
Publication
Marcoleta et al., 2018 - Evaluating Different Virulence Traits of Klebsiella pneumoniae Using Dictyostelium discoideum and Zebrafish Larvae as Host Models.
Other Figures
All Figure Page
Back to All Figure Page
Fig. 5

K. pneumoniae can infect zebrafish larvae by immersion and colonize its gastrointestinal tract. (A) Scheme of the gastrointestinal tract of a 5-dpf zebrafish larva, showing three arbitrarily defined zones (anterior, middle and posterior). (B) Semi-quantitative assessment of the intestinal colonization upon immersing zebrafish larvae in a suspension containing different K. pneumoniae strains or with E. coli DH5α. At the indicated time, larvae were anesthetized, observed under a fluorescence stereomicroscope, and the total intestinal colonization was scored in a scale from 1 to 9, following the criteria described in the Materials and Methods section. The data presented correspond to six replicates with a total of 75 larvae per bacterial strain. (C) Fluorescence microphotographs of zebrafish larvae representative of the data showed in (B). Bright field images of representative non-infected larvae at 48 and 96 h post-immersion are presented for more clarity. The larvae vasculature is shown in green, while infecting bacteria are shown in red. Scale bar, 500 μm. ****p < 0.0001.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Infect Microbiol